Reproduction Abstracts

Introduction: The α-1,3-galactosyltransferase knockout (GalT KO) pig is used as the major model for xenotransplantation studies since the knockout of the α-1,3-galactosyl epitope makes pig organ transplantation successful in primates. The homozygous GalT KO pig is produced by a combination of nuclear transfer technique and mating with a heterozygous GalT KO pig. Since most studies using GalT KO pigs have analyzed only the immunological features of their cells, we...

Introduction: Forkhead box protein N1 (FoxN1) regulates development, differentiation, and function of thymus epithelial cells (TECs), both in the prenatal and postnatal thymus. In mice, knockout of the FoxN1 results in two well-known defects as hairlessness and athymia. However, knockout of FoxN1 in other species has not been discovered yet. RNA-guided endonucleases (RGENs), derived from the prokaryotic type II CRISPR-Cas system, enable targeted specific genes. We successfully...